Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p012 | Testis | WCRB2014

Mitochondrial activity and reactive oxygen species production define distinct subpopulations of human sperm with different functional properties

Ramalho-Santos Joao , Marques Monica , Paiva Artur , Almeida-Santos Teresa , Sousa Ana Paula

Human ejaculates are heterogeneous, and can be comprised of different subpopulations. We have shown that mitochondrial activity varied within ejaculates, and that sperm with high mitochondrial activity were more functionally relevant. Mitochondria are also known to produce reactive oxygen species (ROS), which have been implicated both in sperm function, and in causing gamete dysfunction, depending on amounts and timing of production. We have applied several fluorescent probes ...